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COMT Kit ELISA

COMT Reactivité: Humain AA 52-271 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN2859257
  • Antigène Voir toutes COMT Kits ELISA
    COMT (Catechol-O-Methyltransferase (COMT))
    Épitope
    AA 52-271
    Reactivité
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human COMT
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    E.coli, G52-P271
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: G52-P271
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Brain, liver, placenta, lymphocytes and erythrocytes.

    Plaque
    Pre-coated
    Protocole
    human COMT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for COMT has been precoated onto 96-well plates. Standards (E.coli, G52-P271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human COMT amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human COMT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human COMT standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 305, Standard deviation: 15.55, CV(%): 5.1
    • Sample 2: n=16, Mean(pg/ml): 818, Standard deviation: 43.35, CV(%): 5.3
    • Sample 3: n=16, Mean(pg/ml): 1249, Standard deviation: 77.43, CV(%): 6.2,
    • Sample 1: n=24, Mean(pg/ml): 254, Standard deviation: 16.25, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 699, Standard deviation: 38.44, CV(%): 5.5
    • Sample 3: n=24, Mean(pg/ml): 1358, Standard deviation: 92.34, CV(%): 6.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes COMT Kits ELISA
    COMT (Catechol-O-Methyltransferase (COMT))
    Autre désignation
    COMT (COMT Produits)
    Synonymes
    Comt1 Kit ELISA, D16Wsu103e Kit ELISA, D330014B15Rik Kit ELISA, COMT Kit ELISA, mb-comt Kit ELISA, s-comt Kit ELISA, comt Kit ELISA, MGC154444 Kit ELISA, zgc:114157 Kit ELISA, si:dkey-13a21.15 Kit ELISA, zK13A21.15 Kit ELISA, zgc:162236 Kit ELISA, catechol-O-methyltransferase Kit ELISA, microRNA 4761 Kit ELISA, catechol-O-methyltransferase S homeolog Kit ELISA, methyltransferase Kit ELISA, catechol-o-methyltransferase Kit ELISA, catechol-O-methyltransferase a Kit ELISA, catechol-O-methyltransferase b Kit ELISA, catechol O-methyltransferase Kit ELISA, COMT Kit ELISA, Comt Kit ELISA, MIR4761 Kit ELISA, comt Kit ELISA, comt.2 Kit ELISA, comt.S Kit ELISA, Rv1703c Kit ELISA, Mb1729c Kit ELISA, comta Kit ELISA, comtb Kit ELISA, LOC100354142 Kit ELISA
    Sujet

    Protein Function: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

    Background: Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).

    Synonyms: Catechol O-methyltransferase,2.1.1.6,COMT,

    Full Gene Name: Catechol O-methyltransferase

    Cellular Localisation: Isoform Soluble: Cytoplasm.
    ID gène
    1312
    UniProt
    P21964
    Pathways
    Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome
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