COMT Kit ELISA

N° du produit ABIN6720158

Détail du produit COMT Kit ELISA

Antigène: COMT (Catechol-O-Methyltransferase (COMT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Catechol O methyltransferase, Catechol O-methyltransferase, COMT, COMT_HUMAN, EC 2.1.1.6

Background: Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).

Gene Name: COMT

Production: Natural and recombinant human COMT. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: G52-P271

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human COMT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human COMT standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: COMT (Catechol-O-Methyltransferase (COMT))

Autre désignation: COMT (COMT Produits)

Synonymes: Comt1 Kit ELISA, D16Wsu103e Kit ELISA, D330014B15Rik Kit ELISA, COMT Kit ELISA, mb-comt Kit ELISA, s-comt Kit ELISA, comt Kit ELISA, MGC154444 Kit ELISA, zgc:114157 Kit ELISA, si:dkey-13a21.15 Kit ELISA, zK13A21.15 Kit ELISA, zgc:162236 Kit ELISA, catechol-O-methyltransferase Kit ELISA, microRNA 4761 Kit ELISA, catechol-O-methyltransferase S homeolog Kit ELISA, methyltransferase Kit ELISA, catechol-o-methyltransferase Kit ELISA, catechol-O-methyltransferase a Kit ELISA, catechol-O-methyltransferase b Kit ELISA, catechol O-methyltransferase Kit ELISA, COMT Kit ELISA, Comt Kit ELISA, MIR4761 Kit ELISA, comt Kit ELISA, comt.2 Kit ELISA, comt.S Kit ELISA, Rv1703c Kit ELISA, Mb1729c Kit ELISA, comta Kit ELISA, comtb Kit ELISA, LOC100354142 Kit ELISA

ID gène: 1312

NCBI Accession: NP_000745

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome