CD86 Kit ELISA

N° du produit ABIN2859285

Ce kit ELISA Souris CD86 est un kit ELISA Colorimetric conçu pour quantifier Souris CD86.

Aperçu rapide pour CD86 Kit ELISA (ABIN2859285)

Antigène: CD86

Épitope: AA 26-245

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit CD86 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD86/B7-2

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: NSO, V26-G245

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: V26-G245

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed on activated B-cells.

Plaque: Pre-coated

Protocole: mouse CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD86 has been precoated onto 96-well plates. Standards (NSO, V26-G245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD86 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD86 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD86 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.21, Standard deviation: 0.011, CV(%): 5.3
• Sample 2: n=16, Mean(ng/ml): 1.44, Standard deviation: 0.084, CV(%): 5.9
• Sample 3: n=16, Mean(ng/ml): 2.59, Standard deviation: 0.161, CV(%): 6.2,
• Sample 1: n=24, Mean(ng/ml): 0.23, Standard deviation: 0.013, CV(%): 5.8
• Sample 2: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.095, CV(%): 6.5
• Sample 3: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.150, CV(%): 6.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CD86

Autre désignation: CD86

Sujet:

Protein Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.

Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Synonyms: T-lymphocyte activation antigen CD86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1,CD86,Cd86,

Full Gene Name: T-lymphocyte activation antigen CD86

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

ID gène: 12524

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation