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ATF2 Kit ELISA

ATF2 Reactivité: Humain Colorimetric DNA-Binding ELISA Cell Extracts, Tissue Samples
N° du produit ABIN2866259
  • Antigène Voir toutes ATF2 Kits ELISA
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Reactivité
    • 12
    • 10
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    DNA-Binding ELISA
    Application
    ELISA
    Fonction
    DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
    Marque
    TransAM®
    Type d'échantillon
    Cell Extracts, Tissue Samples
    Analytical Method
    Quantitative
    Specificité
    TransAM ATF-2 Kits are tested for sensitivity in detecting ATF-2 activation.
    Attributs du produit
    Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
    TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.
    Ingrédients
    One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    Nuclear extracts prepared from untreated and treated HeLa (Anisomycin) cells are diluted to 0.625 μg/well and assayed using the TransAM ATF-2 Kit. The ratio of the signals from the treated cells over the untreated must be above 4. Lot No. 25209003 was developed for 7 minutes. It gave a ratio of 4.1 (Figure 1). The endogenous level of ATF-2 expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM ATF-2 Kits are also tested for specificity in detecting ATF-2 activity. TransAM ATF-2 assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated ATF-2 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents ATF-2 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on ATF-2 binding.

    Durée du test
    5 h
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C/-20 °C/-80 °C
    Stockage commentaire
    Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.
    Date de péremption
    6 months
  • Antigène Voir toutes ATF2 Kits ELISA
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Autre désignation
    Atf-2 (ATF2 Produits)
    Synonymes
    CRE-BP1 Kit ELISA, CREB2 Kit ELISA, HB16 Kit ELISA, TREB7 Kit ELISA, CG12850 Kit ELISA, CG3749 Kit ELISA, dATF-2 Kit ELISA, DmelCG30420 Kit ELISA, CG30420 Kit ELISA, CHN1 Kit ELISA, Atf-2 Kit ELISA, CRE-BP Kit ELISA, Creb2 Kit ELISA, D130078H02Rik Kit ELISA, D18875 Kit ELISA, Tg(Gzma-Klra1)7Wum Kit ELISA, mXBP Kit ELISA, atf2-A Kit ELISA, atf2l Kit ELISA, activating transcription factor 2 Kit ELISA, Activating transcription factor-2 Kit ELISA, activating transcription factor 2 S homeolog Kit ELISA, ATF2 Kit ELISA, Atf-2 Kit ELISA, Atf2 Kit ELISA, atf2.S Kit ELISA, atf2 Kit ELISA
    Pathways
    Signalisation MAPK, Signalisation RTK, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
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