IL-3 Kit ELISA

N° du produit ABIN3198578

Kit ELISA IL-3 Humain, Colorimetric test pour la quantification de Humain IL-3.

Aperçu rapide pour IL-3 Kit ELISA (ABIN3198578)

Antigène: IL-3 (Interleukin 3 (IL-3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.63 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit IL-3 Kit ELISA

Seuil minimal de détection: 15.63 pg/mL

Fonction: This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

Analytical Method: Quantitative

Specificité: IL-3 ELISA Kit, Human

Ingrédients:

• Plate
• Standard
• Diluent

Matériel non inclus: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: protect your eyes

Stock: 4 °C

Stockage commentaire: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Détail du antigène

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL-3

Sujet: Interleukin 3 (IL-3) encoded by the IL3 gene is a cytokine secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., erythropoietin (EPO), granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6.

ID gène: 3562

NCBI Accession: NM_000588

UniProt: P08700

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy