Interferon gamma Kit ELISA

N° du produit ABIN411282

Détail du produit Interferon gamma Kit ELISA

Antigène: Interferon gamma (IFNG)

Épitope: AA 24-166

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IFN gamma

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: Q24-Q166

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: Q24-Q166

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the type II (or gamma) interferon family.

Tissue Specificity: Released primarily from activated T lymphocytes.

Plaque: Pre-coated

Protocole: human IFN gamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IFN gamma has been precoated onto 96-well plates. Standards(E.coli, Q24-Q166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFN gamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IFN gamma amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IFN gamma standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IFN gamma standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 98.5, Standard deviation: 5.32, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 264, Standard deviation: 9.24, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 579, Standard deviation: 22.00, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 107, Standard deviation: 8.667, CV(%): 8.1
• Sample 2: n=24, Mean(pg/ml): 315, Standard deviation: 20.79, CV(%): 6.6
• Sample 3: n=24, Mean(pg/ml): 589, Standard deviation: 31.81, CV(%): 5.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Interferon gamma Kit ELISA (ABIN411282)

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Détail du antigène

Antigène: Interferon gamma (IFNG)

Autre désignation: IFNG (IFNG Produits)

Synonymes: IFG Kit ELISA, IFI Kit ELISA, IFN-g Kit ELISA, Ifg Kit ELISA, IFNG2 Kit ELISA, IFN-gamma Kit ELISA, IFN-G Kit ELISA, IFNG Kit ELISA, IFNgamma Kit ELISA, TCRalpha Kit ELISA, INF-G Kit ELISA, ifng Kit ELISA, interferon gamma Kit ELISA, interferon, gamma 1-2 Kit ELISA, IFNG Kit ELISA, Ifng Kit ELISA, ifng1-2 Kit ELISA

Sujet:

Protein Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Background: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Synonyms: Interferon gamma,IFN-gamma,Immune interferon,IFNG,

Full Gene Name: Interferon gamma

Cellular Localisation: Secreted.

ID gène: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy