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IGFBP3 Kit ELISA

IGFBP3 Reactivité: Humain AA 28-291 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN411290
  • Antigène Voir toutes IGFBP3 Kits ELISA
    IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
    Épitope
    AA 28-291
    Reactivité
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP-3
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: A28Q-291
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: A28Q-291
    Top Product
    Discover our top product IGFBP3 Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    human IGFBP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-3 has been precoated onto 96-well plates. Standards(NSO, A28Q-291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-3 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human IGFBP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP-3 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.12, Standard deviation: 0.058, CV(%): 5.2
    • Sample 2: n=16, Mean(ng/ml): 3.41, Standard deviation: 0.198, CV(%): 5.8
    • Sample 3: n=16, Mean(ng/ml): 5.23, Standard deviation: 0.241, CV(%): 4.6,
    • Sample 1: n=24, Mean(ng/ml): 1.24, Standard deviation: 0.077, CV(%): 6.2
    • Sample 2: n=24, Mean(ng/ml): 3.26, Standard deviation: 0.231, CV(%): 7.1
    • Sample 3: n=24, Mean(ng/ml): 6.34, Standard deviation: 0.552, CV(%): 8.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Altas, Kuzu, Arpaci, Unal, Can, Barut, Kokturk, Ilikhan, Bayraktaroglu: "The Clinical Values of Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor Binding Protein-3 Levels in Blood and Thyroid Nodules." dans: International journal of endocrinology, Vol. 2017, pp. 3145234, (2017) (PubMed).

    Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." dans: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

  • Antigène Voir toutes IGFBP3 Kits ELISA
    IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
    Autre désignation
    IGFBP3 (IGFBP3 Produits)
    Synonymes
    BP-53 Kit ELISA, IBP3 Kit ELISA, AI649005 Kit ELISA, IGFBP-3 Kit ELISA, IGgfbp3 Kit ELISA, IGF-BP3 Kit ELISA, zgc:91788 Kit ELISA, IGFBP3 Kit ELISA, igfbp3 Kit ELISA, insulin like growth factor binding protein 3 Kit ELISA, insulin-like growth factor binding protein 3 Kit ELISA, IGFBP3 Kit ELISA, Igfbp3 Kit ELISA, igfbp3 Kit ELISA, IGFBP-3 Kit ELISA, LOC100305016 Kit ELISA
    Sujet

    Background: Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29 kDa. As a result of glycosylation, the molecular mass is 41KDa.

    Synonyms: cDNA FLJ31712 fis, clone NT2RI2006445, highly similar to Insulin-like growth factor-binding protein 3 ,cDNA FLJ34387 fis, clone HCHON1000191, highly similar to Insulin-like growth factor-binding protein 3 ,cDNA FLJ38550 fis, clone HCHON2002123, highly similar to Insulin-like growth factor-binding protein 3 ,cDNA PSEC0177 fis, clone OVARC1000287, highly similar to Insulin-like growth factor-binding protein 3 ,

    Full Gene Name: insulin-like growth factor binding protein 3

    ID gène
    3486
    UniProt
    B3KPF0
    Pathways
    Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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