IL-3 Kit ELISA

N° du produit ABIN411298

Kit ELISA IL-3 Souris, Colorimetric test pour la quantification de Souris IL-3 et a été mentionné dans 2+ publications.

Aperçu rapide pour IL-3 Kit ELISA (ABIN411298)

Antigène: IL-3 (Interleukin 3 (IL-3))

Épitope: AA 27-166

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit IL-3 Kit ELISA

Seuil minimal de détection: 7.8 pg/mL

Fonction: Sandwich Mouse IL-3 ELISA Kit to quantitate Mouse Il3 in cell culture supernatants and serum.

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A27-C166

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.2%, 5.2%, 6.4%
Inter-Assay Precision (CV%): 7.6%, 5.6%, 8.2%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour IL-3 Kit ELISA (ABIN411298)

Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." dans: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." dans: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

Détail du antigène

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL3

Sujet:

Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15KDa.

Gene Full Name: interleukin 3

ID gène: 16187

UniProt: P01586

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy