CCL2 Kit ELISA

N° du produit ABIN411320

Détail du produit CCL2 Kit ELISA

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Épitope: AA 24-99

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MCP-1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: Q24-T99

Réactivité croisée (Details): There is cross-reactivity with human Eotaxin, MCP-3<1 % .

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: Q24-T99

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Plaque: Pre-coated

Protocole: human MCP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MCP-1 has been precoated onto 96-well plates. Standards(E.coli, 24-T99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MCP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MCP-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MCP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MCP-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 73, Standard deviation: 2.7, CV(%): 3.7
• Sample 2: n=16, Mean(pg/ml): 362, Standard deviation: 17.74, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 663, Standard deviation: 34.5, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 93, Standard deviation: 5.022, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 397, Standard deviation: 24.22, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 695, Standard deviation: 51.43, CV(%): 7.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CCL2 Kit ELISA (ABIN411320)

Namlı Kalem, Akgun, Kalem, Bakirarar, Celik: "Chemokine (C-C motif) ligand-2 (CCL2) and oxidative stress markers in recurrent pregnancy loss and repeated implantation failure." dans: Journal of assisted reproduction and genetics, Vol. 34, Issue 11, pp. 1501-1506, (2018) (PubMed).

Sun, Chen, Wang, Wan, Zhang, Zhang, Lin, Zhang: "Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma." dans: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6905217, (2018) (PubMed).

Capistrano, Meneses, de Oliveira Neves, de Almeida Leitão, Martins, Libório: "Renal Evaluation in Common Variable Immunodeficiency." dans: Journal of immunology research, Vol. 2018, pp. 5841031, (2018) (PubMed).

Blanchard, Stepanovska, Starck, Erhardt, Römer, Meyer Zu Heringdorf, Pfeilschifter, Zangemeister-Wittke, Huwiler: "Downregulation of the S1P Transporter Spinster Homology Protein 2 (Spns2) Exerts an Anti-Fibrotic and Anti-Inflammatory Effect in Human Renal Proximal Tubular Epithelial Cells." dans: International journal of molecular sciences, Vol. 19, Issue 5, (2018) (PubMed).

Liao, Cai, Xu, Wang, Qiu, Xie, Huang, Sui: "Protective Role of Antioxidant Huskless Barley Extracts on TNF-α-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells." dans: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3846029, (2018) (PubMed).

Bao, Li, Luo, Tang, Lv, Li, Zhou: "NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation." dans: Mediators of inflammation, Vol. 2016, pp. 5308170, (2017) (PubMed).

Sun, Zhu, Cai, Qiu: "Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway." dans: International journal of molecular sciences, Vol. 18, Issue 4, (2017) (PubMed).

Chen, Liu, Chen, Tseng, Shen, Hwang, Hsu: "Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression." dans: Scientific reports, Vol. 7, Issue 1, pp. 11864, (2017) (PubMed).

Liu, Li, Liu, Wang, Jia: "Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide." dans: International journal of ophthalmology, Vol. 9, Issue 1, pp. 9-14, (2016) (PubMed).

Aizman, Vinodkumar, McGrogan, Bates: "Cell Injury-Induced Release of Fibroblast Growth Factor 2: Relevance to Intracerebral Mesenchymal Stromal Cell Transplantations." dans: Stem cells and development, Vol. 24, Issue 14, pp. 1623-34, (2015) (PubMed).

Jiang, Zhang, Xiong, Jia, Sun, Lin, Shen, Xie, Yan: "EETs Attenuate Ox-LDL-Induced LTB4 Production and Activity by Inhibiting p38 MAPK Phosphorylation and 5-LO/BLT1 Receptor Expression in Rat Pulmonary Arterial Endothelial Cells." dans: PLoS ONE, Vol. 10, Issue 6, pp. e0128278, (2015) (PubMed).

Tu, Chen, Yang, Qi, Lu, Zhao: "Amyloid-? Activates Microglia and Regulates Protein Expression in a Manner Similar to Prions." dans: Journal of molecular neuroscience : MN, Vol. 56, Issue 2, pp. 509-18, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." dans: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Han, Wang, Zhang, Liu, Li, Wang, Song, Pang, Ouyang, Tang: "Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NF?B pathways." dans: Biology open, Vol. 4, Issue 5, pp. 661-5, (2015) (PubMed).

Dai, Wu, He, Zhang, Chen, Xu, Wang, Gruzdev, Zeldin, Wang: "Epoxyeicosatrienoic acids regulate macrophage polarization and prevent LPS-induced cardiac dysfunction." dans: Journal of cellular physiology, Vol. 230, Issue 9, pp. 2108-19, (2015) (PubMed).

Li, Wang, Zhang, Ma, Zhao: "IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells." dans: BMC cancer, Vol. 14, pp. 480, (2015) (PubMed).

Liu, Wang, Ding, Wang, Zeng: "A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-α activity." dans: International journal of molecular medicine, Vol. 34, Issue 3, pp. 725-32, (2015) (PubMed).

Meneses, Libório, de Daher, da Silva, da Costa, Pontes, Martins: "Urinary monocyte chemotactic protein-1 (MCP-1) in leprosy patients: increased risk for kidney damage." dans: BMC infectious diseases, Vol. 14, pp. 451, (2014) (PubMed).

Xu, Kong, Zhou, Zhang, Liu, Yan, Xie, Xie: "oxLDL/?2GPI/anti-?2GPI complex induced macrophage differentiation to foam cell involving TLR4/NF-kappa B signal transduction pathway." dans: Thrombosis research, Vol. 134, Issue 2, pp. 384-92, (2014) (PubMed).

Tu, Yang, Zhou, Qi, Wang, Kouadir, Xu, Yin, Zhao: "PrP106-126 and A? 1-42 peptides induce BV-2 microglia chemotaxis and proliferation." dans: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 107-16, (2014) (PubMed).

Détail du antigène

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: CCL2 (CCL2 Produits)

Synonymes: GDCF-2 Kit ELISA, HC11 Kit ELISA, HSMCR30 Kit ELISA, MCAF Kit ELISA, MCP-1 Kit ELISA, MCP1 Kit ELISA, SCYA2 Kit ELISA, SMC-CF Kit ELISA, AI323594 Kit ELISA, JE Kit ELISA, Scya2 Kit ELISA, Sigje Kit ELISA, MCP-1A Kit ELISA, MCP1A Kit ELISA, C-C motif chemokine ligand 2 Kit ELISA, chemokine (C-C motif) ligand 2 Kit ELISA, C-C motif chemokine 2 Kit ELISA, CCL2 Kit ELISA, Ccl2 Kit ELISA, LOC101120093 Kit ELISA

Sujet:

Protein Function: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

Background: Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Synonyms: C-C motif chemokine 2,HC11,Monocyte chemoattractant protein 1,Monocyte chemotactic and activating factor,MCAF,Monocyte chemotactic protein 1,MCP-1,Monocyte secretory protein JE,Small-inducible cytokine A2,CCL2,MCP1, SCYA2,

Full Gene Name: C-C motif chemokine 2

Cellular Localisation: Secreted.

ID gène: 6347

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2