Growth Hormone 1 Kit ELISA

N° du produit ABIN411378

Détail du produit Growth Hormone 1 Kit ELISA

Antigène: Growth Hormone 1 (GH1)

Épitope: AA 27-217

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Growth Hormone

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: F27-F217

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: F27-F217

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human GH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GH has been precoated onto 96-well plates. Standards(E.coli, F27-F217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GH amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human GH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human GH standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 101, Standard deviation: 4.44, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 335, Standard deviation: 12.06, CV(%): 3.6
• Sample 3: n=16, Mean(pg/ml): 618, Standard deviation: 24.10, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 9.922, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 354, Standard deviation: 26.55, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 596, Standard deviation: 43.51, CV(%): 7.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Growth Hormone 1 (GH1)

Autre désignation: GH1 (GH1 Produits)

Synonymes: GH Kit ELISA, GH-N Kit ELISA, GHN Kit ELISA, IGHD1B Kit ELISA, hGH-N Kit ELISA, Gh1 Kit ELISA, GH1 Kit ELISA, gh Kit ELISA, Gh Kit ELISA, GH2 Kit ELISA, RNGHGP Kit ELISA, ghl Kit ELISA, gh-n Kit ELISA, ghn Kit ELISA, ighd1b Kit ELISA, gh1 Kit ELISA, ghb-A Kit ELISA, GHI Kit ELISA, GHB3 Kit ELISA, growth hormone 1 Kit ELISA, growth hormone Kit ELISA, somatotropin-like Kit ELISA, somatotropin Kit ELISA, growth hormone prepeptide Kit ELISA, growth hormone 1 L homeolog Kit ELISA, growth hormone 1 S homeolog Kit ELISA, Somatotropin-1 Kit ELISA, GH1 Kit ELISA, Gh Kit ELISA, GH Kit ELISA, LOC100305005 Kit ELISA, Gh1 Kit ELISA, LOC100534452 Kit ELISA, LOC100232594 Kit ELISA, LOC100303681 Kit ELISA, gh1 Kit ELISA, LOC100356068 Kit ELISA, LOC100136588 Kit ELISA, gh1.L Kit ELISA, gh1.S Kit ELISA, LOC109081196 Kit ELISA

Sujet:

Background: Growth hormone(GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone(GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency(GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.

Synonyms: Growth hormone 1 isoform 1 ,HCG1749481, isoform CRA_s ,GH1 ,hCG_1749481 ,

Full Gene Name: growth hormone 1

Cellular Localisation: Secreted.

ID gène: 2688

UniProt: B1A4G6

Pathways: Signalisation NF-kappaB, Signalistation JAK/STAT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process