CGRP Kit ELISA

N° du produit ABIN414387

Le kit ELISA CGRP (ABIN414387) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain CGRP. Ce kit ELISA a été cité dans 13 publications.

Aperçu rapide pour CGRP Kit ELISA (ABIN414387)

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 6.17 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit CGRP Kit ELISA

Seuil minimal de détection: 6.17 pg/mL

Fonction: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CGRP in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP).
No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.

Sensibilité: < 2.23 pg/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour CGRP Kit ELISA (ABIN414387)

Leira, Ameijeira, Domínguez, López-Arias, Ávila-Gómez, Pérez-Mato, Sobrino, Campos, DAiuto, Leira, Blanco: "Periodontal inflammation is related to increased serum calcitonin gene-related peptide levels in patients with chronic migraine." dans: Journal of periodontology, Vol. 90, Issue 10, pp. 1088-1095, (2020) (PubMed).

Tarperi, Sanchis-Gomar, Montagnana, Danese, Salvagno, Gelati, Skroce, Schena, Lippi: "Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headache." dans: Clinical chemistry and laboratory medicine, Vol. 58, Issue 10, pp. 1707-1712, (2020) (PubMed).

Pérez-Pereda, Toriello-Suárez, Ocejo-Vinyals, Guiral-Foz, Castillo-Obeso, Montes-Gómez, Martínez-Nieto, Iglesias, González-Quintanilla, Oterino: "Serum CGRP, VIP, and PACAP usefulness in migraine: a case-control study in chronic migraine patients in real clinical practice." dans: Molecular biology reports, Vol. 47, Issue 9, pp. 7125-7138, (2020) (PubMed).

Pinto-Sanchez, Hall, Ghajar, Nardelli, Bolino, Lau, Martin, Cominetti, Welsh, Rieder, Traynor, Gregory, De Palma, Pigrau, Ford, Macri, Berger, Bergonzelli, Surette, Collins, Moayyedi, Bercik: "Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome." dans: Gastroenterology, Vol. 153, Issue 2, pp. 448-459.e8, (2017) (PubMed).

Lei, Zhu, Zhang, Duan, Lei, Huang: "Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction." dans: Cardiology, Vol. 134, Issue 4, pp. 436-43, (2016) (PubMed).

McDonald, Smith, Smith, Changli Xue, Golianu, Cripps: "Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis." dans: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 116, Issue 6, pp. 497-505, (2016) (PubMed).

Riesco, Cernuda-Morollón, Martínez-Camblor, Pérez-Alvarez, Verano, García-Cabo, Serrano-Pertierra, Pascual: "Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients." dans: Cephalalgia : an international journal of headache, (2016) (PubMed).

Cernuda-Morollón, Ramón, Martínez-Camblor, Serrano-Pertierra, Larrosa, Pascual: "OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine." dans: Pain, Vol. 156, Issue 5, pp. 820-4, (2015) (PubMed).

Cernuda-Morollón, Martínez-Camblor, Ramón, Larrosa, Serrano-Pertierra, Pascual: "CGRP and VIP levels as predictors of efficacy of Onabotulinumtoxin type A in chronic migraine." dans: Headache, Vol. 54, Issue 6, pp. 987-95, (2014) (PubMed).

Sambataro, Seganfreddo, Canal, Furlan, Del Pup, Niero, Paccagnella, Gherlinzoni, Dei Tos: "Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot." dans: International journal of vascular medicine, Vol. 2014, pp. 589412, (2014) (PubMed).

Yadav, Yadav, Goel, Singh, Natu, Negi: "Calcitonin gene- and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case-control study." dans: Archives of gynecology and obstetrics, Vol. 290, Issue 5, pp. 897-903, (2014) (PubMed).

Lee, Cho, An, Na, Park, Kim, Lee, Kim, Kim, Jang, Kim: "Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2012, pp. 190370, (2011) (PubMed).

Salas Arruz, Martínez-Vargas: "[Acute toxicity of the glandular venom of Loxosceles sp. in albino mice]." dans: Boletín chileno de parasitología, Vol. 41, Issue 3-4, pp. 43-51, (1987) (PubMed).

Détail du antigène

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Autre désignation: Calcitonin Gene Related Peptide (CGRP)

Sujet: CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta

UniProt: P10092

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour