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MAP2K3 Kit ELISA

MAP2K3 Reactivité: Humain, Souris, Rat pSer189 Colorimetric Cell ELISA Cell Culture Cells
N° du produit ABIN4881290
  • Antigène Voir toutes MAP2K3 Kits ELISA
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    Épitope
    pSer189
    Reactivité
    Humain, Souris, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based Human/Mouse/Rat MKK3 (S189) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
    Type d'échantillon
    Cell Culture Cells
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibodies provided in this kit recognizes human/mouse/rat MKK3 phosphorylated at Serine-189 and total MKK3 for comparison.
    Attributs du produit
    • Site and signal pathway-specific
    • In vitro detection of adherent cell culture
    • No sample lysis needed
    • Compatible with a standard ELISA plate reader
    • Faster results than with ELISA
    • Adaptable for high-throughput screening and drug discovery
    Ingrédients
    • uncoated 96-well Microplate
    • Wash Buffer A
    • Wash Buffer B
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Volume d'échantillon
    100 μL
    Plaque
    Uncoated
    Protocole
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes MAP2K3 Kits ELISA
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    Autre désignation
    MKK3 (MAP2K3 Produits)
    Synonymes
    MAPKK3 Kit ELISA, MEK3 Kit ELISA, MKK3 Kit ELISA, PRKMK3 Kit ELISA, SAPKK-2 Kit ELISA, SAPKK2 Kit ELISA, AW212142 Kit ELISA, Prkmk3 Kit ELISA, mMKK3b Kit ELISA, Mkk3 Kit ELISA, mitogen-activated protein kinase kinase 3 Kit ELISA, mitogen activated protein kinase kinase 3 Kit ELISA, MAP kinase activator XMEK3 Kit ELISA, MAP2K3 Kit ELISA, Map2k3 Kit ELISA, map2k3 Kit ELISA
    ID gène
    5606
    UniProt
    P46734
    Pathways
    Signalisation MAPK, Signalisation TLR, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2
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