TGFA Kit ELISA

N° du produit ABIN4881554

Le kit ELISA TGFA (ABIN4881554) Chien est un kit ELISA Colorimetric conçu pour quantifier Chien TGFA.

Aperçu rapide pour TGFA Kit ELISA (ABIN4881554)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Détail du produit TGFA Kit ELISA

Fonction: Canine (dog) Transforming Growth Factor alpha (TGFA) ELISA Kit.

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Canine Transforming Growth Factor alpha (TGFA)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μl to 1 μl volumes
  • Adjustable 1-25 μl pipettes for reagent preparation
  • 100 μl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  ,
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μl to 1 μl volumes
    • Adjustable 1-25 μl pipettes for reagent preparation
    • 100 μl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2^° to 8^°C from the date of shipment. Standard (recombinant protein) should be stored at -20^°C or -80^°C (recommended at -80^°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2^° to 8^°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20^°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF alpha

Sujet: Gene Symbols: Transforming growth factor alpha

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway