ARNT Kit ELISA

N° du produit ABIN4881888

Détail du produit ARNT Kit ELISA

Antigène: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human ARNT/HIF-1 beta ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Autre désignation: ARNT (ARNT Produits)

Synonymes: HIF-1-beta Kit ELISA, HIF-1beta Kit ELISA, HIF1-beta Kit ELISA, HIF1B Kit ELISA, HIF1BETA Kit ELISA, TANGO Kit ELISA, bHLHe2 Kit ELISA, D3Ertd557e Kit ELISA, Drnt Kit ELISA, ESTM42 Kit ELISA, Hif1b Kit ELISA, W08714 Kit ELISA, mKIAA4051 Kit ELISA, Arnt1 Kit ELISA, ARNT Kit ELISA, zgc:136664 Kit ELISA, arnt1 Kit ELISA, hif1b Kit ELISA, tango Kit ELISA, xArnt Kit ELISA, hif1beta Kit ELISA, hif-1beta Kit ELISA, arnt.2 Kit ELISA, MGC68805 Kit ELISA, aryl hydrocarbon receptor nuclear translocator Kit ELISA, aryl hydrocarbon receptor nuclear translocator S homeolog Kit ELISA, ARNT Kit ELISA, Arnt Kit ELISA, arnt Kit ELISA, arnt.S Kit ELISA

ID gène: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, L'effet Warburg