DDR1 Kit ELISA

N° du produit ABIN4882584

Détail du produit DDR1 Kit ELISA

Antigène: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human DDR1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Autre désignation: DDR1 (DDR1 Produits)

Synonymes: CG11573 Kit ELISA, CG13984 Kit ELISA, CG13985 Kit ELISA, CG33531 Kit ELISA, CG9488 Kit ELISA, CG9490 Kit ELISA, CT33540 Kit ELISA, DDR Kit ELISA, Dmel\\CG33531 Kit ELISA, Dmel_CG13984 Kit ELISA, 6030432F18 Kit ELISA, AI323681 Kit ELISA, CD167a Kit ELISA, Cak Kit ELISA, Nep Kit ELISA, PTK3A Kit ELISA, CAK Kit ELISA, CD167 Kit ELISA, EDDR1 Kit ELISA, HGK2 Kit ELISA, MCK10 Kit ELISA, NEP Kit ELISA, NTRK4 Kit ELISA, PTK3 Kit ELISA, RTK6 Kit ELISA, TRKE Kit ELISA, Drd1 Kit ELISA, PTK3D Kit ELISA, Discoidin domain receptor Kit ELISA, discoidin domain receptor family, member 1 Kit ELISA, discoidin domain receptor tyrosine kinase 1 Kit ELISA, Ddr Kit ELISA, Ddr1 Kit ELISA, DDR1 Kit ELISA

ID gène: 780

UniProt: Q08345

Pathways: Signalisation RTK, Smooth Muscle Cell Migration