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DDR1 Kit ELISA

DDR1 Reactivité: Humain pTyr792 Colorimetric Sandwich ELISA Cell Culture Lysate
N° du produit ABIN5526735
  • Antigène Voir toutes DDR1 Kits ELISA
    DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
    Épitope
    pTyr792, total
    Reactivité
    • 5
    • 2
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit. This assay semi-quantitatively measures DDR1 phosphorylated at Tyrosine-792 as well as total DDR1 in cell lysate samples.
    Type d'échantillon
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human DDR1 phosphorylated at site Tyrosine-792 as well as total DDR1.
    Attributs du produit
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes DDR1 Kits ELISA
    DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
    Autre désignation
    DDR1 (DDR1 Produits)
    Synonymes
    CG11573 Kit ELISA, CG13984 Kit ELISA, CG13985 Kit ELISA, CG33531 Kit ELISA, CG9488 Kit ELISA, CG9490 Kit ELISA, CT33540 Kit ELISA, DDR Kit ELISA, Dmel\\CG33531 Kit ELISA, Dmel_CG13984 Kit ELISA, 6030432F18 Kit ELISA, AI323681 Kit ELISA, CD167a Kit ELISA, Cak Kit ELISA, Nep Kit ELISA, PTK3A Kit ELISA, CAK Kit ELISA, CD167 Kit ELISA, EDDR1 Kit ELISA, HGK2 Kit ELISA, MCK10 Kit ELISA, NEP Kit ELISA, NTRK4 Kit ELISA, PTK3 Kit ELISA, RTK6 Kit ELISA, TRKE Kit ELISA, Drd1 Kit ELISA, PTK3D Kit ELISA, Discoidin domain receptor Kit ELISA, discoidin domain receptor family, member 1 Kit ELISA, discoidin domain receptor tyrosine kinase 1 Kit ELISA, Ddr Kit ELISA, Ddr1 Kit ELISA, DDR1 Kit ELISA
    ID gène
    780
    UniProt
    Q08345
    Pathways
    Signalisation RTK, Smooth Muscle Cell Migration
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