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FRS2 Kit ELISA
FRS2
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882937
Détail du produit FRS2 Kit ELISA
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Antigène
Voir toutes FRS2 Kits ELISA
FRS2
(Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
Reactivité
Toutes les réactivités sur FRS2 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human FRS2 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes .
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes FRS2 Kits ELISA
FRS2
(Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
Autre désignation
FRS2 (FRS2 Produits )
Synonymes
frs2 Kit ELISA, wu:fd21h10 Kit ELISA, zgc:91973 Kit ELISA, FRS2A Kit ELISA, FRS2alpha Kit ELISA, SNT Kit ELISA, SNT-1 Kit ELISA, SNT1 Kit ELISA, 4732458E18 Kit ELISA, C330018A15Rik Kit ELISA, Frs2alpha Kit ELISA, fibroblast growth factor receptor substrate 2 Kit ELISA, fibroblast growth factor receptor substrate 2b Kit ELISA, FRS2 Kit ELISA, frs2b Kit ELISA, Frs2 Kit ELISA
ID gène
10818
UniProt
Q8WU20
Pathways
Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Signaling Events mediated by VEGFR1 and VEGFR2
Vus récemment
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