TPP1 Kit ELISA

N° du produit ABIN4884857

Le kit ELISA TPP1 (ABIN4884857) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain TPP1.

Aperçu rapide pour TPP1 Kit ELISA (ABIN4884857)

Antigène: TPP1 (Tripeptidyl Peptidase I (TPP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 10 pg/mL - 2500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit TPP1 Kit ELISA

Seuil minimal de détection: 10 pg/mL

Fonction: Human Tripeptidyl-peptidase I/TPP1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin- 1 (CT-1), Cathepsin V, FABP1 (L-FABP), FGF-20, GDF-8 (Myostatin), HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2 (Noelin-2), p53, PDECGF (Thymidine Phosphorylase), PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TREM-2, TrkC, TROY (TNFRSF19), Uromodulin, XIAP.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 10 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TPP1 (Tripeptidyl Peptidase I (TPP1))

Autre désignation: Tripeptidyl-Peptidase I

Sujet:

Gene Symbols: TPP1, GIG1, CLN2, UNQ267/PRO304

Protein Name (Synonyms): Tripeptidyl-peptidase 1 (TPP-1) (EC 3.4.14.9) (Cell growth-inhibiting gene 1 protein) (Lysosomal pepstatin-insensitive protease) (LPIC) (Tripeptidyl aminopeptidase) (Tripeptidyl-peptidase I) (TPP-I)

ID gène: 1200

UniProt: O14773

Pathways: Cycle Cellulaire, ER-Nucleus Signaling