Clusterin Kit ELISA
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- Antigène Voir toutes Clusterin (CLU) Kits ELISA
- Clusterin (CLU)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Mouse Clusterin (Apolipoprotein J) ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes mouse Clusterin.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Clusterin (CLU) Kits ELISA
- Clusterin (CLU)
- Autre désignation
- Clusterin (CLU Produits)
- Synonymes
- LOC100136120 Kit ELISA, clu Kit ELISA, MGC53055 Kit ELISA, zgc:73352 Kit ELISA, xclu Kit ELISA, MGC89163 Kit ELISA, CLU Kit ELISA, APO-J Kit ELISA, APOJ Kit ELISA, CLI Kit ELISA, CLU1 Kit ELISA, CLU2 Kit ELISA, KUB1 Kit ELISA, NA1/NA2 Kit ELISA, SGP-2 Kit ELISA, SGP2 Kit ELISA, SP-40 Kit ELISA, TRPM-2 Kit ELISA, TRPM2 Kit ELISA, DAG Kit ELISA, RATTRPM2B Kit ELISA, SP40 Kit ELISA, TRPM2B Kit ELISA, Trpm2 Kit ELISA, Trpmb Kit ELISA, AI893575 Kit ELISA, ApoJ Kit ELISA, Cli Kit ELISA, D14Ucla3 Kit ELISA, Sgp-2 Kit ELISA, Sgp2 Kit ELISA, Sugp-2 Kit ELISA, GP80 Kit ELISA, gpIII Kit ELISA, 40 Kit ELISA, clusterin-1 Kit ELISA, clusterin L homeolog Kit ELISA, clusterin Kit ELISA, Clusterin Kit ELISA, LOC100136120 Kit ELISA, clu.L Kit ELISA, clu Kit ELISA, CLU Kit ELISA, clus Kit ELISA, Clu Kit ELISA, LOC101113728 Kit ELISA
- ID gène
- 12759
- UniProt
- Q06890
- Pathways
- Apoptose, Negative Regulation of intrinsic apoptotic Signaling
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