AXL Kit ELISA (AXL Receptor tyrosine Kinase)

Details for Product AXL Kit ELISA No. ABIN4885698
Antigène
  • JTK11
  • UFO
  • AI323647
  • Ark
  • Tyro7
  • Ufo
  • AXL receptor tyrosine kinase
  • Axl receptor tyrosine kinase
  • AXL
  • Axl
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
6
4
3
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Options
Fonction Custom Mouse AXL SpeedELISA Kit.
Marque SpeedELISA
Type d'échantillon Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Méthode de détection Colorimetric
Attributs du produit
  • Compressed workflow with only one wash step and three incubation steps
  • 3 hours total processing time
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Ingrédients
  • Streptavidin-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Capture Antibody
  • HRP Conjugated Detection Antibody
  • TMB One-Step Substrate
Matériel non inclus
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others AXL products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation AXL (AXL ELISA Kit Extrait)
ID gène 26362
UniProt Q00993
Pathways Signalisation RTK, Cellular Response to Molecule of Bacterial Origin
Volume d'échantillon 100 μL
Durée du test 3 h
Plaque Pre-coated
Protocole
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 70 μL of the Antibody Cocktail to each well.
  3. Incubate 10 minutes at RT.
  4. Add 50 μL of sample or standard to each well.
  5. Incubate 2 h at RT.
  6. Wash Wells
  7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  8. Incubate 30 min at RT.
  9. Add 50 μL of Stop Solution to each well.
  10. Read at 450 nm immediately.
Restrictions For Research Use only
Stock -20 °C
Stockage commentaire May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Date de péremption 6 months
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