IGF1 Kit ELISA

N° du produit ABIN4885921

Détail du produit IGF1 Kit ELISA

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivité: Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rat IGF-1 SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF-1 (IGF1 Produits)

Synonymes: IGF-I Kit ELISA, IGF1A Kit ELISA, IGFI Kit ELISA, C730016P09Rik Kit ELISA, Igf-1 Kit ELISA, Igf-I Kit ELISA, Npt2B Kit ELISA, IGF-1 Kit ELISA, IGFIA Kit ELISA, IGF-IB Kit ELISA, igf1 Kit ELISA, IGF-1L Kit ELISA, IGF-1a Kit ELISA, IGF1 Kit ELISA, igf-1 Kit ELISA, igf1-A Kit ELISA, igf1.S Kit ELISA, xigf1 Kit ELISA, insulin like growth factor 1 Kit ELISA, insulin-like growth factor 1 Kit ELISA, insulin-like growth factor I Kit ELISA, insulin like growth factor 1 L homeolog Kit ELISA, IGF1 Kit ELISA, Igf1 Kit ELISA, LOC100136741 Kit ELISA, igf1 Kit ELISA, LOC678666 Kit ELISA, igf1.L Kit ELISA, LOC104911603 Kit ELISA

ID gène: 24482

UniProt: P08025

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation