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eIF4EBP1 Kit ELISA

EIF4EBP1 Reactivité: Souris, Humain pThr36 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4885954
  • Antigène Voir toutes eIF4EBP1 (EIF4EBP1) Kits ELISA
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Épitope
    pThr36, total
    Reactivité
    • 7
    • 6
    • 5
    • 1
    Souris, Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Mouse Phospho-4E-BP1 (T36) and Total 4E-BP1 ELISA Kit. This assay semi-quantitatively measures phophorylated 4E-BP1 (Thr36) and Total 4E-BP1 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human and mouse 4E-BP1 phosphorylated at site Threonine-36 and total 4E-BP1.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product EIF4EBP1 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes eIF4EBP1 (EIF4EBP1) Kits ELISA
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Autre désignation
    4E-BP1 (EIF4EBP1 Produits)
    Synonymes
    4E-BP1 Kit ELISA, 4EBP1 Kit ELISA, BP-1 Kit ELISA, PHAS-I Kit ELISA, 4e-bp1 Kit ELISA, AA959816 Kit ELISA, 153432_at Kit ELISA, 43-BP Kit ELISA, 4E-BP Kit ELISA, 4EBP Kit ELISA, 4e-bp Kit ELISA, 4eBP Kit ELISA, 4ebp Kit ELISA, BcDNA.HL08053 Kit ELISA, BcDNA:HL08053 Kit ELISA, CG8846 Kit ELISA, CT8705 Kit ELISA, Dm 4E-BP Kit ELISA, Dmel\\CG8846 Kit ELISA, Phas1 Kit ELISA, Thor/EIF4EBP Kit ELISA, anon-WO0172774.158 Kit ELISA, d4E-BP Kit ELISA, d4E-BP1 Kit ELISA, d4EBP Kit ELISA, d4e-bp Kit ELISA, d4eBP Kit ELISA, dthor Kit ELISA, eIF4E-BP Kit ELISA, l(2)06270 Kit ELISA, l(2)k07736 Kit ELISA, l(2)k13517 Kit ELISA, pp20 Kit ELISA, thor Kit ELISA, EIF4EBP1 Kit ELISA, fc04h09 Kit ELISA, wu:fc04h09 Kit ELISA, zgc:64137 Kit ELISA, eukaryotic translation initiation factor 4E binding protein 1 Kit ELISA, CG8846 gene product from transcript CG8846-RA Kit ELISA, EIF4EBP1 Kit ELISA, Eif4ebp1 Kit ELISA, Thor Kit ELISA, eif4ebp1 Kit ELISA
    ID gène
    1978
    UniProt
    Q13541
    Pathways
    Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, BCR Signaling
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