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FGFR2 Kit ELISA
FGFR2
Reactivité: Humain
pTyr
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
N° du produit ABIN4886102
Détail du produit FGFR2 Kit ELISA
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Antigène
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FGFR2
(Fibroblast Growth Factor Receptor 2 (FGFR2))
Épitope
pTyr
Reactivité
Toutes les réactivités sur FGFR2 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human Phosphotyrosine FGFR2 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine FGFR2 in lysate samples.
Type d'échantillon
Cell Lysate, Tissue Lysate
Analytical Method
Semi-Quantitative
Specificité
The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-FGFR2.
Attributs du produit
Rapidly measure phosphorylated protein in lysates Screen numerous different cell lysates without performing a Western Blot analysis Minimal hands-on time, convenient, and non-radioactive material
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Biotinylated Anti-Phosphotyrosine Antibody Stop Solution Assay Diluent(s) Positive Control Sample Lysis Buffer Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water 100 mL and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 μL to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents and samples as instructed in the manual. Add 100 μL of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes FGFR2 Kits ELISA
FGFR2
(Fibroblast Growth Factor Receptor 2 (FGFR2))
Autre désignation
FGFR2 (FGFR2 Produits )
Synonymes
FGFR2 Kit ELISA, FGFR2IIIb Kit ELISA, bek Kit ELISA, bfr-1 Kit ELISA, cd332 Kit ELISA, cek3 Kit ELISA, cfd1 Kit ELISA, ect1 Kit ELISA, fgfr-2 Kit ELISA, jws Kit ELISA, k-sam Kit ELISA, kgfr Kit ELISA, tk14 Kit ELISA, tk25 Kit ELISA, xfgfr2 Kit ELISA, DKFZp469H1521 Kit ELISA, BBDS Kit ELISA, BEK Kit ELISA, BFR-1 Kit ELISA, CD332 Kit ELISA, CEK3 Kit ELISA, CFD1 Kit ELISA, ECT1 Kit ELISA, JWS Kit ELISA, K-SAM Kit ELISA, KGFR Kit ELISA, TK14 Kit ELISA, TK25 Kit ELISA, AU043015 Kit ELISA, AW556123 Kit ELISA, Bek Kit ELISA, Fgfr-2 Kit ELISA, Fgfr-7 Kit ELISA, Fgfr7 Kit ELISA, KGFRTr Kit ELISA, svs Kit ELISA, FGF-R Kit ELISA, fgfr72IIIb Kit ELISA, FGFR Kit ELISA, fc56c05 Kit ELISA, wu:fc56c05 Kit ELISA, fibroblast growth factor receptor 2 Kit ELISA, fibroblast growth factor receptor 2 L homeolog Kit ELISA, FGFR2 Kit ELISA, LOC100084641 Kit ELISA, fgfr2 Kit ELISA, Fgfr2 Kit ELISA, fgfr2.L Kit ELISA
ID gène
2263
UniProt
P21802
Pathways
Signalisation RTK , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Regulation of Muscle Cell Differentiation , Skeletal Muscle Fiber Development , Growth Factor Binding
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