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Neurotrophin 3 Kit CLIA

NTF3 Reactivité: Humain Chemiluminescent Sandwich ELISA 27.4 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN491812
  • Antigène Voir toutes Neurotrophin 3 (NTF3) Kits CLIA
    Neurotrophin 3 (NTF3)
    Reactivité
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Chemiluminescent
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    27.4 pg/mL - 20000 pg/mL
    Seuil minimal de détection
    27.4 pg/mL
    Application
    ELISA
    Fonction
    The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of NT3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité

    This assay has high sensitivity and excellent specificity for detection of Neurotrophin 3 (NT3).
    No significant cross-reactivity or interference between Neurotrophin 3 (NT3) and analogues was observed.

    Réactivité croisée (Details)
    No significant cross-reactivity or interference between Neurotrophin 3 (NT3) and analogues was observed.
    Sensibilité
    10.5 pg/mL
    Ingrédients
    • Pre-coated, ready to use black 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • Substrate A
    • Substrate B
    • Wash Buffer (30 x concentrate)
    • Instruction manual
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  • Indications d'application
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    Commentaires

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Volume d'échantillon
    100 μL
    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The microplate provided in this kit has been pre-coated with an antibody specific to Neurotrophin 3 (NT3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neurotrophin 3 (NT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neurotrophin 3 (NT3) level in the sample or standard.,
    Préparation des réactifs
    1. Bring all kit components and samples to room temperature (18-25 °C) before use.
    2. Standard - Reconstitute the Standard with 0.5 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 20,000pg/mL. Prepare 7 tubes containing 0.6 mL Standard Diluent and produce a triple dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 20,000pg/mL, 6,666.7pg/mL, 2,222.2pg/mL, 740.7pg/mL, 246.9pg/mL, 82.3pg/mL, 27.4pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with Assay Diluent A and B, respectively (1:100).
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. Substrate working Solution - Mix the substrate A and B by the ratio of 99:1 to make the substrate working solution. Mix thoroughly. For example, prepare 1,000μL Substrate working Solution with 990μL Substrate A + 10μL Substrate B.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. Prepare Substrate working Solution within 15 minutes before assay.
    6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or container for reagent preparation will influence the detection result.
    Précision du teste

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Stock
    4 °C
    Stockage commentaire
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Neurotrophin 3 (NTF3) Kits CLIA
    Neurotrophin 3 (NTF3)
    Autre désignation
    Neurotrophin 3 (NT3) (NTF3 Produits)
    Synonymes
    HDNF Kit CLIA, NGF-2 Kit CLIA, NGF2 Kit CLIA, NT3 Kit CLIA, NT-3 Kit CLIA, wu:fl98d04 Kit CLIA, hdnf Kit CLIA, ngf-2 Kit CLIA, ngf2 Kit CLIA, nt-3 Kit CLIA, nt3 Kit CLIA, NTF3 Kit CLIA, AI316846 Kit CLIA, AI835689 Kit CLIA, Nt3 Kit CLIA, Ntf-3 Kit CLIA, neurotrophin 3 Kit CLIA, neurotrophin 3 S homeolog Kit CLIA, neurotrophin 3 L homeolog Kit CLIA, NTF3 Kit CLIA, ntf3 Kit CLIA, ntf3.S Kit CLIA, Ntf3 Kit CLIA, ntf3.L Kit CLIA
    UniProt
    P20783
    Pathways
    Signalisation RTK, Neurotrophin Signaling Pathway
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