Leptin Kit ELISA

N° du produit ABIN4987005

Ce kit ELISA Humain Leptin est un kit ELISA Colorimetric conçu pour quantifier Humain Leptin.

Aperçu rapide pour Leptin Kit ELISA (ABIN4987005)

Antigène: Leptin (LEP)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Détail du produit Leptin Kit ELISA

Seuil minimal de détection: 31.25 pg/mL

Analytical Method: Quantitative

Specificité: Natural and recombinantHuman Leptin Ligand

Sensibilité: 7 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Leptin (LEP)

Autre désignation: Leptin

Sujet: Human Leptin (gene name OB) is a 16 kDa, 146 amino acid (aa) residue, non-glycosylated polypeptide that regulates adipose tissue mass and energy balance (1 - 6). Mature human Leptin shares 87 % and 84 % aa identity with mouse and rat Leptin, respectively(1,7). Human Leptin is active in both the mouse and rat systems (8, 9). Leptin is expressed almost exclusively by adipocytes and its production is influenced by hormones, cytokines and nutrients (5, 7, 10) and circulates in the plasma, crosses the blood-brain barrier, and is present in human breast milk (3 - 6, 11). The human Leptin receptor (designated ObR or LEPR) is a 150 kDa, 1144 aa residue, type I transmembrane glycoprotein of the IL-6 receptor family of Class I cytokine receptors (12, 13). The gene for ObR undergoes considerable splicing, forming variants a-d with cytoplasmic domains of variable length, plus the potentially soluble form ObRe (13, 14). The long form, ObRb (formerly OB RL), is expressed mainly in the hypothalamic arcuate nucleus and is essential for signal transduction (6, 15, 16). In a concentration-dependent manner, Leptin signaling can have diverse effects, causing neurons that express pro-opiomelanocortin (POMC) peptides to reduce food intake, and neurons that express neuropeptide Y and agouti-related protein (NpY and AgRP) to increase food intake (4, 6). Leptin is fundamentally a

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process