IL-7 Kit ELISA
Aperçu rapide pour IL-7 Kit ELISA (ABIN5026943)
Antigène
Voir toutes IL-7 (IL7) Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
-
-
Seuil minimal de détection
- 7.8 pg/mL
-
Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-7 concentrations in serum, plasma and other biological fluids.
-
Analytical Method
- Quantitative
-
Sensibilité
- < 4.69 pg/mL
-
Ingrédients
- plate, standard, diluent, antibodies
-
Matériel non inclus
- pipettes, tubes, reader, incubator
-
-
-
-
Volume d'échantillon
- 100 μL
-
Durée du test
- 3 - 4 h
-
Plaque
- Pre-coated
-
Protocole
- This assay employs an antibody specific for Anti-humanIL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-7antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Restrictions
- For Research Use only
-
-
-
Précaution d'utilisation
- protect your eyes
-
Stock
- 4 °C
-
-
- IL-7 (IL7) (Interleukin 7 (IL7))
-
Autre désignation
- IL-7 (Interleukin 7)
-
Pathways
- Signalistation JAK/STAT
Antigène Voir toutes IL-7 (IL7) Kits ELISA
-