LTA Kit ELISA

N° du produit ABIN5026953

Kit ELISA LTA Humain, Colorimetric test pour la quantification de Humain LTA.

Aperçu rapide pour LTA Kit ELISA (ABIN5026953)

Antigène: LTA (Lymphotoxin-alpha (LTA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit LTA Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate.

Analytical Method: Quantitative

Sensibilité: 9.4 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human TNFBeta concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFBeta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNFBeta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFBeta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: LTA (Lymphotoxin-alpha (LTA))

Autre désignation: TNF Beta (Tumor Necrosis Factor Beta)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process