Bcl-2 Kit ELISA

N° du produit ABIN5026957

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Bcl-2.

Aperçu rapide pour Bcl-2 Kit ELISA (ABIN5026957)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Bcl-2 Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensibilité: < 0.094 ng/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: Bcl -2 (B cell Lymphoma 2)

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling