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IL-13 Kit ELISA

IL13 Reactivité: Humain Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum
N° du produit ABIN5026963
  • Antigène Voir toutes IL-13 (IL13) Kits ELISA
    IL-13 (IL13) (Interleukin 13 (IL13))
    Reactivité
    • 12
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    This immunoassay kit allows for the in vitro quantitative determination of Human IL-13 concentrations in serum, plasma and other biological fluids.
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensibilité
    < 9.4 pg/mL
    Ingrédients
    plate, standard, diluent, antibodies
    Matériel non inclus
    pipettes, tubes, reader, incubator
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  • Volume d'échantillon
    100 μL
    Durée du test
    3 - 4 h
    Plaque
    Pre-coated
    Protocole
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-13 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-13 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-13 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-13 can be calculated using the standard curve.
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    protect your eyes
    Stock
    4 °C
  • Antigène Voir toutes IL-13 (IL13) Kits ELISA
    IL-13 (IL13) (Interleukin 13 (IL13))
    Autre désignation
    IL-13 (Interleukin 13) (IL13 Produits)
    Synonymes
    IL13 Kit ELISA, IL-13 Kit ELISA, P600 Kit ELISA, Il-13 Kit ELISA, L13 Kit ELISA, interleukin 13 Kit ELISA, IL13 Kit ELISA, Il13 Kit ELISA
    Pathways
    Signalistation JAK/STAT, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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