Leptin Kit ELISA

N° du produit ABIN5068068

Kit ELISA Humain Leptin, test Colorimetric pour la quantification de Humain Leptin.

Aperçu rapide pour Leptin Kit ELISA (ABIN5068068)

Antigène: Leptin (LEP)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Plasma, Biological Buffers, Cell Culture Supernatant, Serum

Détail du produit Leptin Kit ELISA

Fonction: This Human Leptin ELISA kit is to be used for the in vitro quantitative determination of leptin in human serum, plasma, buffered solution or cell culture medium. The assay recognizes both natural and recombinant forms of leptin.

Analytical Method: Quantitative

Réactivité croisée (Details): Calculated cross reactivity: Hu

Sensibilité: > 3.5 pg/mL

Attributs du produit: Leptin, Human (Ob, Obese Protein, Obesity Factor) ELISA Kit

Ingrédients:

• 96 Test Kit 192 Test Kit
• Standard &
• 25,&
• 25, 2 vials 4 vials
• Standard Diluent 1x60ml 2x60ml
• Microtiter Plate 1x96wells 2x96wells
• Mab (Biotin) 1x11ml 2x11ml
• Streptavidin (HRP), 100X 1x125ul 2x125ul
• HRP Diluent 1x25ml 1x25ml
• Wash Buffer, 25X 1x100ml 1x100ml
• TMB 1x25ml 1x25ml
• Stop Solution 1x25ml 1x25ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• This Human Leptin kit is a solid phase sandwich ELISA. A monoclonal antibody specific for human leptin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known leptin content, control specimens, and unknowns, are pipetted into these wells followed by addition of a second biotinylated monoclonal antibody. During the first incubation, the leptin antigen binds to the immobilized (capture) antibody on one site and to the solution phase biotinylated antibody on a second site. After removal of excess secondary antibody, Streptavidin-Peroxidase enzyme is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of leptin present in the original specimen.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C

Détail du antigène

Antigène: Leptin (LEP)

Autre désignation: Leptin

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process