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TBK1 Kit ELISA

TBK1 Reactivité: Humain, Souris, Rat pSer172 Colorimetric Cell ELISA Adherent Cell Culture
N° du produit ABIN5526719
  • Antigène Voir toutes TBK1 Kits ELISA
    TBK1 (TANK-Binding Kinase 1 (TBK1))
    Épitope
    pSer172, total
    Reactivité
    Humain, Souris, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based ELISA Kit. This assay semi-quantitatively measures TBK1 phosphorylated at Serine-172 as well as total TBK1 in adherent cell lines.
    Type d'échantillon
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human, Mouse and Rat TBK1 phosphorylated at site Serine-172 as well as total TBK1.
    Attributs du produit
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Ingrédients
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes TBK1 Kits ELISA
    TBK1 (TANK-Binding Kinase 1 (TBK1))
    Autre désignation
    TBK1 (TBK1 Produits)
    Synonymes
    nak Kit ELISA, t2k Kit ELISA, TBK1 Kit ELISA, wu:fk70c05 Kit ELISA, zgc:136548 Kit ELISA, NAK Kit ELISA, T2K Kit ELISA, 1200008B05Rik Kit ELISA, AI462036 Kit ELISA, AW048562 Kit ELISA, TANK binding kinase 1 L homeolog Kit ELISA, TANK binding kinase 1 Kit ELISA, TANK-binding kinase 1 Kit ELISA, tbk1.L Kit ELISA, TBK1 Kit ELISA, tbk1 Kit ELISA, Tbk1 Kit ELISA
    ID gène
    29110
    UniProt
    Q9UHD2, Q9WUN2, D4A7D3
    Pathways
    Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome
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