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RICTOR Kit ELISA

RICTOR Reactivité: Humain, Souris pThr1135 Colorimetric Cell ELISA Adherent Cell Culture
N° du produit ABIN5526752
  • Antigène Voir toutes RICTOR Kits ELISA
    RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))
    Épitope
    pThr1135, total
    Reactivité
    Humain, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based ELISA Kit. This assay semi-quantitatively measures Rictor phosphorylated at Threonine-1135 as well as total Rictor in adherent cell lines.
    Type d'échantillon
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human and Mouse Rictor phosphorylated at site Threonine-1135 as well as total Rictor.
    Attributs du produit
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Ingrédients
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product RICTOR Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes RICTOR Kits ELISA
    RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))
    Autre désignation
    Rictor (RICTOR Produits)
    Synonymes
    PIA Kit ELISA, mAVO3 Kit ELISA, 4921505C17Rik Kit ELISA, 6030405M08Rik Kit ELISA, AVO3 Kit ELISA, AW492497 Kit ELISA, D530039E11Rik Kit ELISA, Fyb Kit ELISA, RPTOR independent companion of MTOR complex 2 Kit ELISA, RPTOR independent companion of MTOR, complex 2 Kit ELISA, RICTOR Kit ELISA, Rictor Kit ELISA
    ID gène
    253260
    UniProt
    Q6R327, Q6QI06
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events
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