PDGFD Kit ELISA

N° du produit ABIN5526788

Kit ELISA PDGFD Humain, Colorimetric test pour la quantification de Humain PDGFD.

Aperçu rapide pour PDGFD Kit ELISA (ABIN5526788)

Antigène: PDGFD (Platelet Derived Growth Factor D (PDGFD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 12.5 pg/mL - 800 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Détail du produit PDGFD Kit ELISA

Seuil minimal de détection: 12.5 pg/mL

Fonction: Sandwich ELISA for quantitative detection of Human PDGF-DD concentrations.

Analytical Method: Quantitative

Sensibilité: 5 pg/mL

Ingrédients:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Matériel non inclus:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Date de péremption: 6-12 months

Détail du antigène

Antigène: PDGFD (Platelet Derived Growth Factor D (PDGFD))

Autre désignation: PDGF-DD

Sujet: Alternative Names: IEGF, SCDGFB, Platelet-derived growth factor D, PDGF-D, Iris-expressed growth factor, Spinal cord-derived growth factor B, SCDGF-B

ID gène: 80310

UniProt: Q9GZP0

Pathways: Signalisation RTK, Platelet-derived growth Factor Receptor Signaling