AIF Kit ELISA

N° du produit ABIN5651957

Détail du produit AIF Kit ELISA

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Apoptosis Inducing Factor (AIF). No significant cross-reactivity or interference between Apoptosis Inducing Factor (AIF) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Inducing Factor (AIF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Inducing Factor (AIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Inducing Factor (AIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptosis Inducing Factor (AIF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Autre désignation: Apoptosis Inducing Factor (AIFM1 Produits)

Synonymes: AIF Kit ELISA, CMTX4 Kit ELISA, COWCK Kit ELISA, COXPD6 Kit ELISA, PDCD8 Kit ELISA, CG7263 Kit ELISA, DmAIF Kit ELISA, Dmel\\CG7263 Kit ELISA, GB16024 Kit ELISA, DDBDRAFT_0187853 Kit ELISA, DDBDRAFT_0191137 Kit ELISA, DDB_0187853 Kit ELISA, DDB_0191137 Kit ELISA, aif Kit ELISA, pdcd8 Kit ELISA, AIFM1 Kit ELISA, PCD8 Kit ELISA, AIFsh2 Kit ELISA, Hq Kit ELISA, Pdcd8 Kit ELISA, mAIF Kit ELISA, Aif Kit ELISA, zgc:91994 Kit ELISA, apoptosis inducing factor mitochondria associated 1 Kit ELISA, allograft inflammatory factor 1 Kit ELISA, Apoptosis inducing factor Kit ELISA, apoptosis-inducing factor 1, mitochondrial Kit ELISA, apoptosis inducing factor Kit ELISA, apoptosis inducing factor, mitochondria associated 1 Kit ELISA, apoptosis-inducing factor, mitochondrion-associated, 1 Kit ELISA, apoptosis-inducing factor, mitochondrion-associated 1 Kit ELISA, AIFM1 Kit ELISA, AIF1 Kit ELISA, AIF Kit ELISA, LOC412212 Kit ELISA, aif Kit ELISA, aifm1 Kit ELISA, Aifm1 Kit ELISA

Sujet:

Gene Name: Apoptosis Inducing Factor

Gene Aliases: PDCD8, Programmed Cell Death protein 8, Apoptosis-inducing factor 1, mitochondrial

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, L'effet Warburg