APAF1 Kit ELISA

N° du produit ABIN5651962

Détail du produit APAF1 Kit ELISA

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Apoptotic Peptidase Activating Factor 1 (APAF1). No significant cross-reactivity or interference between Apoptotic Peptidase Activating Factor 1 (APAF1) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptotic Peptidase Activating Factor 1 (APAF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptotic Peptidase Activating Factor 1 (APAF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apoptotic Peptidase Activating Factor 1 (APAF1 Produits)

Synonymes: APAF1 Kit ELISA, ARK Kit ELISA, Apaf-1 Kit ELISA, Apaf1 Kit ELISA, CG6829 Kit ELISA, D-Apaf-1 Kit ELISA, DARK Kit ELISA, Dapaf-1 Kit ELISA, Dapaf-1/HAC-1 Kit ELISA, Dark Kit ELISA, Dark/Apaf-I Kit ELISA, Dark/Dapaf-1/HAC1 Kit ELISA, Dark/Hac-1/dApaf-1 Kit ELISA, Dark/Hac-1/dApaf1 Kit ELISA, Dmel\\CG6829 Kit ELISA, Hac-1 Kit ELISA, Hac-1/Dark Kit ELISA, Hac1 Kit ELISA, T1 Kit ELISA, anon-53Fa Kit ELISA, apaf-1 Kit ELISA, apaf1 Kit ELISA, arc Kit ELISA, ark Kit ELISA, dAPAF-1 Kit ELISA, dApaf-1 Kit ELISA, dApaf-1/DARK/HAC-1 Kit ELISA, dApaf1 Kit ELISA, dArk Kit ELISA, dapaf Kit ELISA, dapaf-1 Kit ELISA, dapaf-1L Kit ELISA, dapaf-1S Kit ELISA, dark Kit ELISA, dark/dapaf-1/hac-1 Kit ELISA, dark/hac-1/dapaf-1 Kit ELISA, hac-1 Kit ELISA, hac1 Kit ELISA, l(2)SH0173 Kit ELISA, APAF-1 Kit ELISA, CED4 Kit ELISA, 6230400I06Rik Kit ELISA, Apaf1l Kit ELISA, fog Kit ELISA, mKIAA0413 Kit ELISA, zgc:136543 Kit ELISA, Death-associated APAF1-related killer Kit ELISA, apoptotic peptidase activating factor 1 L homeolog Kit ELISA, apoptotic peptidase activating factor 1 Kit ELISA, Dark Kit ELISA, apaf1.L Kit ELISA, apaf1 Kit ELISA, APAF1 Kit ELISA, Apaf1 Kit ELISA

Sujet:

Gene Name: Apoptotic Peptidase Activating Factor 1

Gene Aliases: CED4, Apoptotic Protease Activating Factor

ID gène: 317

UniProt: O14727

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity