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BCAR1 Kit ELISA

BCAR1 Reactivité: Humain Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5652342
  • Antigène Voir toutes BCAR1 Kits ELISA
    BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))
    Reactivité
    • 7
    • 4
    • 4
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78.12 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78.12 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). No significant cross-reactivity or interference between Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) and analogues was observed.
    Sensibilité
    27 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 1 (BCAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes BCAR1 Kits ELISA
    BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))
    Autre désignation
    Breast Cancer Anti-Estrogen Resistance 1 (BCAR1 Produits)
    Synonymes
    CAS Kit ELISA, CAS1 Kit ELISA, CASS1 Kit ELISA, CRKAS Kit ELISA, P130Cas Kit ELISA, AI385681 Kit ELISA, Cas Kit ELISA, Crkas Kit ELISA, P130CAS Kit ELISA, MGC68466 Kit ELISA, cas Kit ELISA, p130cas Kit ELISA, BCAR1 Kit ELISA, fc17f11 Kit ELISA, wu:fc17f11 Kit ELISA, zgc:175192 Kit ELISA, BCAR1, Cas family scaffolding protein Kit ELISA, breast cancer anti-estrogen resistance 1 Kit ELISA, breast cancer anti-estrogen resistance 1 L homeolog Kit ELISA, breast cancer anti-estrogen resistance protein 1 Kit ELISA, BCAR1 Kit ELISA, Bcar1 Kit ELISA, bcar1.L Kit ELISA, bcar1 Kit ELISA, LOC100435586 Kit ELISA
    Sujet

    Gene Name: Breast Cancer Anti-Estrogen Resistance 1

    Gene Aliases: CAS, CRKAS, P130Cas, CASS1, Crk-Associated Substrate, Cas Scaffolding Protein Family Member 1

    ID gène
    9564
    UniProt
    P56945
    Pathways
    EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling
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