BRCA1 Kit ELISA

N° du produit ABIN5652344

Détail du produit BRCA1 Kit ELISA

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Breast Cancer Susceptibility Protein 1 (BRCA1). No significant cross-reactivity or interference between Breast Cancer Susceptibility Protein 1 (BRCA1) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Susceptibility Protein 1 (BRCA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Susceptibility Protein 1 (BRCA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Susceptibility Protein 1 (BRCA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Susceptibility Protein 1 (BRCA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 1 (BRCA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 1 (BRCA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: Breast Cancer Susceptibility Protein 1 (BRCA1 Produits)

Synonymes: BRCAI Kit ELISA, BRCC1 Kit ELISA, BROVCA1 Kit ELISA, IRIS Kit ELISA, PNCA4 Kit ELISA, PPP1R53 Kit ELISA, PSCP Kit ELISA, RNF53 Kit ELISA, iris Kit ELISA, pscp Kit ELISA, brcai Kit ELISA, brcc1 Kit ELISA, rnf53 Kit ELISA, brca1 Kit ELISA, BRCA1 Kit ELISA, LOC100224649 Kit ELISA, BRCA1, DNA repair associated Kit ELISA, breast cancer 1 Kit ELISA, breast cancer 1, early onset Kit ELISA, breast cancer 1 L homeolog Kit ELISA, BRCA1 Kit ELISA, brca1 Kit ELISA, LOC755086 Kit ELISA, Brca1 Kit ELISA, brca1.L Kit ELISA

Sujet:

Gene Name: Breast Cancer Susceptibility Protein 1

Gene Aliases: BRCAI, BRCC1, IRIS, PSCP, RNF53, Breast Cancer 1,Early Onset, RING finger protein 53

ID gène: 672

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus