CGRP Kit CLIA

N° du produit ABIN5652477

Détail du produit CGRP Kit CLIA

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Competition ELISA

Gamme de detection: 7.81 pg/mL - 2000 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.

Sensibilité: 2.77 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP). A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Calcitonin Gene Related Peptide (CGRP) level in the sample or standard.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Autre désignation: Calcitonin Gene Related Peptide (CALCA Produits)

Synonymes: CALC1 Kit CLIA, CGRP Kit CLIA, CGRP-I Kit CLIA, CGRP1 Kit CLIA, CT Kit CLIA, KC Kit CLIA, CALC Kit CLIA, CALCA Kit CLIA, CALC3 Kit CLIA, ci-ct Kit CLIA, calca Kit CLIA, CA Kit CLIA, CGRP-1 Kit CLIA, Calc Kit CLIA, Calc1 Kit CLIA, Cgrp Kit CLIA, Ct Kit CLIA, Ctn Kit CLIA, calcitonin Kit CLIA, CAL6 Kit CLIA, Cal1 Kit CLIA, RATCAL6 Kit CLIA, CGRPI Kit CLIA, calc Kit CLIA, cgrp Kit CLIA, zgc:92886 Kit CLIA, calcitonin related polypeptide alpha Kit CLIA, calcitonin related polypeptide beta Kit CLIA, calcitonin-related polypeptide alpha Kit CLIA, calcitonin Kit CLIA, calcitonin/calcitonin-related polypeptide, alpha Kit CLIA, calcitonin-related polypeptide beta Kit CLIA, calcitonin gene-related peptide 2 Kit CLIA, CALCA Kit CLIA, CALCB Kit CLIA, ci-ct Kit CLIA, calca Kit CLIA, Calca Kit CLIA, LOC100008771 Kit CLIA

Sujet:

Gene Name: Calcitonin Gene Related Peptide

Gene Aliases: CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta

UniProt: Q99MP3

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour