CGRP Kit ELISA

N° du produit ABIN5652478

Détail du produit CGRP Kit ELISA

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 12.35 pg/mL - 1000 pg/mL

Seuil minimal de détection: 12.35 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.

Sensibilité: 4.68 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Autre désignation: Calcitonin Gene Related Peptide (CALCA Produits)

Synonymes: CALC1 Kit ELISA, CGRP Kit ELISA, CGRP-I Kit ELISA, CGRP1 Kit ELISA, CT Kit ELISA, KC Kit ELISA, CALC Kit ELISA, CALCA Kit ELISA, CALC3 Kit ELISA, ci-ct Kit ELISA, calca Kit ELISA, CA Kit ELISA, CGRP-1 Kit ELISA, Calc Kit ELISA, Calc1 Kit ELISA, Cgrp Kit ELISA, Ct Kit ELISA, Ctn Kit ELISA, calcitonin Kit ELISA, CAL6 Kit ELISA, Cal1 Kit ELISA, RATCAL6 Kit ELISA, CGRPI Kit ELISA, calc Kit ELISA, cgrp Kit ELISA, zgc:92886 Kit ELISA, calcitonin related polypeptide alpha Kit ELISA, calcitonin related polypeptide beta Kit ELISA, calcitonin-related polypeptide alpha Kit ELISA, calcitonin Kit ELISA, calcitonin/calcitonin-related polypeptide, alpha Kit ELISA, calcitonin-related polypeptide beta Kit ELISA, calcitonin gene-related peptide 2 Kit ELISA, CALCA Kit ELISA, CALCB Kit ELISA, ci-ct Kit ELISA, calca Kit ELISA, Calca Kit ELISA, LOC100008771 Kit ELISA

Sujet:

Gene Name: Calcitonin Gene Related Peptide

Gene Aliases: CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta

UniProt: Q99MP3

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour