Calreticulin Kit CLIA

N° du produit ABIN5652528

Détail du produit Calreticulin Kit CLIA

Antigène: Calreticulin (CALR)

Reactivité: Rat

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calreticulin (CRT). No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.

Sensibilité: 1.21 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Calreticulin (CRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Calreticulin (CRT) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calreticulin (CRT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calreticulin (CRT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Calreticulin (CALR)

Autre désignation: Calreticulin (CALR Produits)

Synonymes: CRT Kit CLIA, RO Kit CLIA, SSA Kit CLIA, cC1qR Kit CLIA, crc Kit CLIA, crt Kit CLIA, Calregulin Kit CLIA, CG9429 Kit CLIA, Clr Kit CLIA, Dmel\\CG9429 Kit CLIA, anon-EST:Liang-2.11 Kit CLIA, anon-EST:Liang-2.17 Kit CLIA, anon-EST:fe3D7 Kit CLIA, clone 2.11 Kit CLIA, clone 2.17 Kit CLIA, eth Kit CLIA, potp Kit CLIA, cc1qr Kit CLIA, ssa Kit CLIA, NV19007 Kit CLIA, CRP55 Kit CLIA, ERp60 Kit CLIA, HACBP Kit CLIA, CHOT1 Kit CLIA, CHT1 Kit CLIA, CT1 Kit CLIA, AA589632 Kit CLIA, CRTR Kit CLIA, Creat Kit CLIA, CCDS1 Kit CLIA, calrl2 Kit CLIA, fc66d10 Kit CLIA, wu:fc66d10 Kit CLIA, wu:fj50c03 Kit CLIA, zgc:66153 Kit CLIA, calreticulin Kit CLIA, calreticulin L homeolog Kit CLIA, Calreticulin Kit CLIA, putative calreticulin Kit CLIA, solute carrier family 6 member 8 Kit CLIA, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 Kit CLIA, CALR Kit CLIA, calr.L Kit CLIA, Crt Kit CLIA, crt Kit CLIA, CRT Kit CLIA, Calr Kit CLIA, calr Kit CLIA, LMJF_31_2600 Kit CLIA, Crc Kit CLIA, Slc6a8 Kit CLIA, SLC6A8 Kit CLIA, LOC8269812 Kit CLIA

Sujet:

Gene Name: Calreticulin

Gene Aliases: CALR, RO, SSA, CC1qR, ERp60, HACBP, grp60, CRTC, CRP55, Calregulin, Sicca Syndrome Antigen A, Autoantigen Ro, Endoplasmic reticulum resident protein 60

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response