FLIP Kit ELISA

N° du produit ABIN5652640

Détail du produit FLIP Kit ELISA

Antigène: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of CASP8 And FADD Like Apoptosis Regulator (CFLAR). No significant cross-reactivity or interference between CASP8 And FADD Like Apoptosis Regulator (CFLAR) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP8 And FADD Like Apoptosis Regulator (CFLAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CASP8 And FADD Like Apoptosis Regulator (CFLAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CASP8 And FADD Like Apoptosis Regulator (CFLAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CASP8 And FADD Like Apoptosis Regulator (CFLAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CASP8 And FADD Like Apoptosis Regulator (CFLAR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CASP8 And FADD Like Apoptosis Regulator (CFLAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Autre désignation: CASP8 And FADD Like Apoptosis Regulator (CFLAR Produits)

Synonymes: Clarp1 Kit ELISA, cflar Kit ELISA, CFLAR Kit ELISA, FLIP Kit ELISA, CASH Kit ELISA, CASP8AP1 Kit ELISA, CLARP Kit ELISA, Casper Kit ELISA, FLAME Kit ELISA, FLAME-1 Kit ELISA, FLAME1 Kit ELISA, I-FLICE Kit ELISA, MRIT Kit ELISA, c-FLIP Kit ELISA, c-FLIPL Kit ELISA, c-FLIPR Kit ELISA, c-FLIPS Kit ELISA, 2310024N18Rik Kit ELISA, A430105C05Rik Kit ELISA, Cash Kit ELISA, ENSMUSG00000072980 Kit ELISA, Flip Kit ELISA, Gm9845 Kit ELISA, c-Flip Kit ELISA, CASP8 and FADD-like apoptosis regulator a Kit ELISA, CASP8 and FADD like apoptosis regulator Kit ELISA, CASP8 and FADD like apoptosis regulator S homeolog Kit ELISA, CASP8 and FADD-like apoptosis regulator Kit ELISA, cflara Kit ELISA, CFLAR Kit ELISA, cflar Kit ELISA, cflar.S Kit ELISA, Cflar Kit ELISA

Sujet:

Gene Name: CASP8 And FADD Like Apoptosis Regulator

Gene Aliases: CASH, CASP8AP1, CLARP, Casper, FLAME1, FLIP, I-FLICE, MRIT, USURPIN, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, Caspase-eight-related protein, FADD-like antiapoptotic molecule 1

Pathways: Apoptose, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development