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Caspase 7 Kit ELISA

CASP7 Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5652659
  • Antigène Voir toutes Caspase 7 (CASP7) Kits ELISA
    Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
    Reactivité
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Caspase 7 (CASP7). No significant cross-reactivity or interference between Caspase 7 (CASP7) and analogues was observed.
    Sensibilité
    0.054 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 7 (CASP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 7 (CASP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 7 (CASP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 7 (CASP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 7 (CASP7) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 7 (CASP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Caspase 7 (CASP7) Kits ELISA
    Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
    Autre désignation
    Caspase 7 (CASP7 Produits)
    Synonymes
    xCaspase-7 Kit ELISA, caspase-7 Kit ELISA, caspase7 Kit ELISA, cmh-1 Kit ELISA, ice-lap3 Kit ELISA, mch3 Kit ELISA, CASP7 Kit ELISA, AI314680 Kit ELISA, CMH-1 Kit ELISA, ICE-IAP3 Kit ELISA, Mch3 Kit ELISA, mCASP-7 Kit ELISA, CASP-7 Kit ELISA, ICE-LAP3 Kit ELISA, LICE2 Kit ELISA, MCH3 Kit ELISA, Caspase-7 Kit ELISA, MCH3/SCA-2 Kit ELISA, SCA-2 Kit ELISA, caspase 7 Kit ELISA, caspase-7 Kit ELISA, caspase 7 S homeolog Kit ELISA, CASP7 Kit ELISA, xcaspase-7 Kit ELISA, casp7 Kit ELISA, casp7.S Kit ELISA, Casp7 Kit ELISA
    Sujet

    Gene Name: Caspase 7

    Gene Aliases: CMH1, ICE-LAP3, MCH3, Caspase 7 Apoptosis-Related Cysteine Peptidase, Cysteine Aspartate Protease 7, Cysteinyl Aspartate Specific Proteinases 7

    ID gène
    15139
    UniProt
    P06684
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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