C3 Kit ELISA

N° du produit ABIN5653222

Détail du produit C3 Kit ELISA

Antigène: C3 (Complement Component 3 (C3))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Complement Component 3 (C3). No significant cross-reactivity or interference between Complement Component 3 (C3) and analogues was observed.

Sensibilité: 1.37 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3 (C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 3 (C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3 (C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 3 (C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 3 (C3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3 (C3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: C3 (Complement Component 3 (C3))

Autre désignation: Complement Component 3 (C3 Produits)

Synonymes: AHUS5 Kit ELISA, ARMD9 Kit ELISA, ASP Kit ELISA, C3a Kit ELISA, C3b Kit ELISA, CPAMD1 Kit ELISA, AI255234 Kit ELISA, HSE-MSF Kit ELISA, Plp Kit ELISA, C3 Kit ELISA, C3-1 Kit ELISA, sb:cb26 Kit ELISA, si:dkey-76b14.4 Kit ELISA, wu:fi34b03 Kit ELISA, wu:fj86e11 Kit ELISA, C3d Kit ELISA, asp Kit ELISA, cpamd1 Kit ELISA, c3 Kit ELISA, complement C3 Kit ELISA, complement component 3 Kit ELISA, complement component c3a, duplicate 1 Kit ELISA, complement component 3 L homeolog Kit ELISA, complement component C3 Kit ELISA, complement C3-like Kit ELISA, C3 Kit ELISA, c3a.1 Kit ELISA, c3.L Kit ELISA, LOC443475 Kit ELISA, c3 Kit ELISA, c3-1 Kit ELISA, LOC100060539 Kit ELISA

Sujet:

Gene Name: Complement Component 3

Gene Aliases: ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein

ID gène: 24232

UniProt: P01026

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling