Cyclin D1 Kit ELISA
-
- Antigène Voir toutes Cyclin D1 (CCND1) Kits ELISA
- Cyclin D1 (CCND1)
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Cyclin D1 (CCND1). No significant cross-reactivity or interference between Cyclin D1 (CCND1) and analogues was observed.
- Sensibilité
- 0.119 ng/mL
- Featured
- Discover our best selling CCND1 Kit ELISA
- Top Product
- Discover our top product CCND1 Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin D1 (CCND1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin D1 (CCND1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin D1 (CCND1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin D1 (CCND1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin D1 (CCND1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin D1 (CCND1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes Cyclin D1 (CCND1) Kits ELISA
- Cyclin D1 (CCND1)
- Autre désignation
- Cyclin D1 (CCND1 Produits)
- Synonymes
- BCL1 Kit ELISA, D11S287E Kit ELISA, PRAD1 Kit ELISA, U21B31 Kit ELISA, AI327039 Kit ELISA, CycD1 Kit ELISA, Cyl-1 Kit ELISA, bcl-1 Kit ELISA, cD1 Kit ELISA, cb161 Kit ELISA, cycd1 Kit ELISA, etID37810.7 Kit ELISA, fb52e01 Kit ELISA, fc45c08 Kit ELISA, fc83a12 Kit ELISA, id:ibd1198 Kit ELISA, wu:fb52e01 Kit ELISA, wu:fc45c08 Kit ELISA, wu:fc83a12 Kit ELISA, ccnd1b Kit ELISA, prad1 Kit ELISA, ccnd1 Kit ELISA, ccnd1a Kit ELISA, bcl1 Kit ELISA, d11s287e Kit ELISA, u21b31 Kit ELISA, CCND1 Kit ELISA, cyclin D1 Kit ELISA, cyclin D1 S homeolog Kit ELISA, cyclin D1 L homeolog Kit ELISA, CCND1 Kit ELISA, Ccnd1 Kit ELISA, ccnd1 Kit ELISA, ccnd1.S Kit ELISA, ccnd1.L Kit ELISA
- Sujet
-
Gene Name: Cyclin D1
Gene Aliases: CCN-D1, BCL1, PRAD1, U21B31, G1/S-Specific Cyclin-D1, Parathyroid Adenomatosis 1, B-Cell CLL/Lymphoma 1, G1/S-Specific Cyclin D1, PRAD1 oncogene
- ID gène
- 595
- UniProt
- P24385
- Pathways
- Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
-