CTH Kit ELISA

N° du produit ABIN5653444

Détail du produit CTH Kit ELISA

Antigène: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cystathionine Gamma Lyase (CSE). No significant cross-reactivity or interference between Cystathionine Gamma Lyase (CSE) and analogues was observed.

Sensibilité: 0.053 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystathionine Gamma Lyase (CSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystathionine Gamma Lyase (CSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystathionine Gamma Lyase (CSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystathionine Gamma Lyase (CSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystathionine Gamma Lyase (CSE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystathionine Gamma Lyase (CSE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Autre désignation: Cystathionine Gamma Lyase (CTH Produits)

Synonymes: DYT17 Kit ELISA, DYT18 Kit ELISA, DYT9 Kit ELISA, EIG12 Kit ELISA, GLUT Kit ELISA, GLUT1 Kit ELISA, GLUT1DS Kit ELISA, HTLVR Kit ELISA, PED Kit ELISA, wu:fb48e03 Kit ELISA, wu:fb58d08 Kit ELISA, zgc:66001 Kit ELISA, zgc:85785 Kit ELISA, CTH Kit ELISA, 0610010I13Rik Kit ELISA, AI314617 Kit ELISA, BC019483 Kit ELISA, CSE Kit ELISA, Cys3 Kit ELISA, cysA Kit ELISA, SCQ11.03c Kit ELISA, DDBDRAFT_0190313 Kit ELISA, DDBDRAFT_0191318 Kit ELISA, DDB_0190313 Kit ELISA, DDB_0191318 Kit ELISA, H Kit ELISA, MGC75946 Kit ELISA, cystathionine gamma-lyase Kit ELISA, solute carrier family 2 member 1 Kit ELISA, cystathionase (cystathionine gamma-lyase) Kit ELISA, cystathionine gamma-lyase L homeolog Kit ELISA, CTH Kit ELISA, SLC2A1 Kit ELISA, cth Kit ELISA, Cth Kit ELISA, SCO3920 Kit ELISA, mlr1566 Kit ELISA, TA_RS00415 Kit ELISA, MAP_RS15640 Kit ELISA, SPO3344 Kit ELISA, CNN01730 Kit ELISA, Mmcs_1928 Kit ELISA, RHA1_RS31820 Kit ELISA, HNE_1212 Kit ELISA, MAV_3867 Kit ELISA, MSMEG_2394 Kit ELISA, Mvan_2142 Kit ELISA, SACE_6136 Kit ELISA, Mflv_4220 Kit ELISA, cysA Kit ELISA, cth.L Kit ELISA

Sujet:

Gene Name: Cystathionine Gamma Lyase

Gene Aliases: CTH, Cystathionase, Cysteine-protein sulfhydrase, Gamma-cystathionase

ID gène: 24962

UniProt: P18757

Pathways: ER-Nucleus Signaling, L'effet Warburg