ERN1 Kit ELISA

N° du produit ABIN5653844

Détail du produit ERN1 Kit ELISA

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1). No significant cross-reactivity or interference between Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Autre désignation: Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1 Produits)

Synonymes: IRE1 Kit ELISA, IRE1P Kit ELISA, IRE1a Kit ELISA, hIRE1p Kit ELISA, 9030414B18Rik Kit ELISA, AI225830 Kit ELISA, C85377 Kit ELISA, Ire1a Kit ELISA, Ire1alpha Kit ELISA, Ire1p Kit ELISA, RGD1559716 Kit ELISA, Ern1 Kit ELISA, Ire1 Kit ELISA, Ire1b Kit ELISA, sb:eu1005 Kit ELISA, zgc:109932 Kit ELISA, endoplasmic reticulum to nucleus signaling 1 Kit ELISA, endoplasmic reticulum to nucleus signaling 2 Kit ELISA, endoplasmic reticulum (ER) to nucleus signalling 1 Kit ELISA, serine/threonine-protein kinase/endoribonuclease IRE1 Kit ELISA, endoplasmic reticulum (ER) to nucleus signalling 2 Kit ELISA, ERN1 Kit ELISA, ERN2 Kit ELISA, Ern1 Kit ELISA, LOC524719 Kit ELISA, Ern2 Kit ELISA, ern1 Kit ELISA

Sujet:

Gene Name: Endoplasmic Reticulum To Nucleus Signalling 1

Gene Aliases: IRE1, IRE1P, Serine/Threonine-Protein Kinase/Endoribonuclease IRE1, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase, Endoribonuclease

ID gène: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response