FGF1 Kit CLIA

N° du produit ABIN5654181

Ce kit ELISA Chemiluminescent est conçu pour la mesure quantitative de Souris FGF1.

Aperçu rapide pour FGF1 Kit CLIA (ABIN5654181)

Antigène: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit FGF1 Kit CLIA

Seuil minimal de détection: 31.25 pg/mL

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1). No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.

Sensibilité: 1.06 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 1, Acidic (FGF1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Autre désignation: Fibroblast Growth Factor 1, Acidic

Sujet:

Gene Name: Fibroblast Growth Factor 1, Acidic

Gene Aliases: ECGF, AFGF, ECGFA, ECGFB, FGF-Alpha, FGFA, HBGF1, ECGFB, HBGF1, Acidic Fibroblast Growth Factor, heparin-binding growth factor 1, Endothelial Cell Growth Factor, Beta

ID gène: 14164

UniProt: P61148

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway