GLA Kit CLIA

N° du produit ABIN5654468

Détail du produit GLA Kit CLIA

Antigène: GLA (Galactosidase, alpha (GLA))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Galactosidase Alpha (GLa). No significant cross-reactivity or interference between Galactosidase Alpha (GLa) and analogues was observed.

Sensibilité: 6.18 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Galactosidase Alpha (GLa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Galactosidase Alpha (GLa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Galactosidase Alpha (GLa) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactosidase Alpha (GLa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactosidase Alpha (GLa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GLA (Galactosidase, alpha (GLA))

Autre désignation: Galactosidase Alpha (GLA Produits)

Synonymes: GALA Kit CLIA, Ags Kit CLIA, zgc:101584 Kit CLIA, MGC130872 Kit CLIA, SMU.877 Kit CLIA, SCF11.21 Kit CLIA, AO090005000217 Kit CLIA, alpha-GAL Kit CLIA, galactosidase alpha Kit CLIA, galactosidase, alpha Kit CLIA, galactosidase alpha S homeolog Kit CLIA, alpha-galactosidase Kit CLIA, aga Kit CLIA, alpha-galactosidase A Kit CLIA, GLA Kit CLIA, Gla Kit CLIA, gla Kit CLIA, gla.S Kit CLIA, agaN Kit CLIA, aga Kit CLIA, agaL Kit CLIA, SCO0541 Kit CLIA, rafA Kit CLIA, melA Kit CLIA, galA Kit CLIA, ANI_1_2528074 Kit CLIA, ANI_1_1502124 Kit CLIA, AOR_1_390174 Kit CLIA, CpipJ_CPIJ002066 Kit CLIA, MCYG_00962 Kit CLIA, MCYG_00791 Kit CLIA, Tsp_02909 Kit CLIA, Tsp_02508 Kit CLIA

Sujet:

Gene Name: Galactosidase Alpha

Gene Aliases: GALA, GL-A, Alpha-D-Galactoside Galactohydrolase, Alpha-D-Galactosidase A, Melibiase, Agalsidase

ID gène: 2717

UniProt: P06280

Pathways: SARS-CoV-2 Protein Interactome