Growth Hormone 1 Kit ELISA

N° du produit ABIN5654885

Détail du produit Growth Hormone 1 Kit ELISA

Antigène: Growth Hormone 1 (GH1)

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 246.9 pg/mL - 20000 pg/mL

Seuil minimal de détection: 246.9 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH). No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.

Sensibilité: 91.4 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone (GH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone (GH) and unlabeled Growth Hormone (GH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone (GH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone (GH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone (GH) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Growth Hormone 1 (GH1)

Autre désignation: Growth Hormone (GH1 Produits)

Synonymes: GH Kit ELISA, GH-N Kit ELISA, GHN Kit ELISA, IGHD1B Kit ELISA, hGH-N Kit ELISA, Gh1 Kit ELISA, GH1 Kit ELISA, gh Kit ELISA, Gh Kit ELISA, GH2 Kit ELISA, RNGHGP Kit ELISA, ghl Kit ELISA, gh-n Kit ELISA, ghn Kit ELISA, ighd1b Kit ELISA, gh1 Kit ELISA, ghb-A Kit ELISA, GHI Kit ELISA, GHB3 Kit ELISA, growth hormone 1 Kit ELISA, growth hormone Kit ELISA, somatotropin-like Kit ELISA, somatotropin Kit ELISA, growth hormone prepeptide Kit ELISA, growth hormone 1 L homeolog Kit ELISA, growth hormone 1 S homeolog Kit ELISA, Somatotropin-1 Kit ELISA, GH1 Kit ELISA, Gh Kit ELISA, GH Kit ELISA, LOC100305005 Kit ELISA, Gh1 Kit ELISA, LOC100534452 Kit ELISA, LOC100232594 Kit ELISA, LOC100303681 Kit ELISA, gh1 Kit ELISA, LOC100356068 Kit ELISA, LOC100136588 Kit ELISA, gh1.L Kit ELISA, gh1.S Kit ELISA, LOC109081196 Kit ELISA

Sujet:

Gene Name: Growth Hormone

Gene Aliases: GH1, GH-N, GHN, hGH-N, Somatotropin, Hygetropin, Jintropin, Kigtropin, Pituitary Growth Hormone, Growth Hormone, Normal

Pathways: Signalisation NF-kappaB, Signalistation JAK/STAT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process