Hexokinase 1 Kit ELISA

N° du produit ABIN5655112

Détail du produit Hexokinase 1 Kit ELISA

Antigène: Hexokinase 1 (HK1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hexokinase 1 (HK1). No significant cross-reactivity or interference between Hexokinase 1 (HK1) and analogues was observed.

Sensibilité: 27.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexokinase 1 (HK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexokinase 1 (HK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexokinase 1 (HK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hexokinase 1 (HK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexokinase 1 (HK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexokinase 1 (HK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Hexokinase 1 (HK1)

Autre désignation: Hexokinase 1 (HK1 Produits)

Synonymes: HK1-ta Kit ELISA, HK1-tb Kit ELISA, HK1-tc Kit ELISA, HKD Kit ELISA, HKI Kit ELISA, HMSNR Kit ELISA, HXK1 Kit ELISA, Hexokinase Kit ELISA, hk1 Kit ELISA, HEX Kit ELISA, HEX-1 Kit ELISA, HK Kit ELISA, ARABIDOPSIS THALIANA HEXOKINASE 1 Kit ELISA, ATHXK1 Kit ELISA, F19B15.160 Kit ELISA, F19B15_160 Kit ELISA, GIN2 Kit ELISA, GLUCOSE INSENSITIVE 2 Kit ELISA, HEXOKINASE Kit ELISA, hexokinase 1 Kit ELISA, im:7148527 Kit ELISA, wu:fc09d08 Kit ELISA, wu:fc16e02 Kit ELISA, wu:fc21e02 Kit ELISA, wu:fq14b11 Kit ELISA, zgc:55790 Kit ELISA, zgc:77618 Kit ELISA, hexokinase-1 Kit ELISA, HK1 Kit ELISA, BB404130 Kit ELISA, Hk-1 Kit ELISA, Hk1-s Kit ELISA, dea Kit ELISA, mHk1-s Kit ELISA, HEXOKIN Kit ELISA, Hxk1 Kit ELISA, NtHxK1 Kit ELISA, NtHxk Kit ELISA, hexokinase Kit ELISA, StHK1 Kit ELISA, hxk Kit ELISA, hk1-A Kit ELISA, hexokinase 1 Kit ELISA, hexokinase-1 Kit ELISA, Hexokinase 1 Kit ELISA, hexokinase 1, putative Kit ELISA, hexokinase 1 L homeolog Kit ELISA, HK1 Kit ELISA, hk1 Kit ELISA, LOC100136279 Kit ELISA, Hex1 Kit ELISA, HXK1 Kit ELISA, Hk1 Kit ELISA, TA19800 Kit ELISA, hxk1 Kit ELISA, LOC107797523 Kit ELISA, LOC102604144 Kit ELISA, hk1.L Kit ELISA

Sujet:

Gene Name: Hexokinase 1

Gene Aliases: HK1-Ta, HK1-Tb, HK1-Tc, HKI, HXK1, Brain form hexokinase

ID gène: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, L'effet Warburg